May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass (By similarity). (www.uniprot.org) Produced in E.coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids.
Cat-Nr: 95036.20
Menge: 20 µg
Preis: 66 €
UniProt ID: Q9TU09 (Protein knowledgebase) Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor. Introduction
A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Description
Leptin Horse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.
The Leptin is purified by proprietary chromatographic techniques. Source
Escherichia Coli. Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Solubility
It is recommended to reconstitute the lyophilized Leptin in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE. Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln. Biological Activity
Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC,using calibrated solution of Leptin Horse as a Reference Standard.
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