Cayman's Pyruvate Assay provides both a fluorescence- and absorbance-based methods for quantifying pyruvate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular pyruvate concentrations in cell culture samples. In the assay,...

Cayman's beta-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying beta-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for beta-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme...

Phenylbutazone is a non-steroidal anti-inflammatory drug and an inhibitor of the peroxidase activity of COX (IC50 = ~100 µM in the presence of hydrogen peroxide). It also inhibits prostaglandin I synthase (IC50 = ~25 µM in the presence of hydrogen peroxide). Phenylbutazone (2 mg/kg) reduces increases in type II...

U-73122 is an inhibitor of PLC-dependent processes, however, the mechanism of action remains unclear. The IC50 values for inhibition of platelet aggregation induced by collagen or thrombin are 0.6 and 5 µM, respectively. It also exhibits inhibitory activity against HIV-1 integrase with an IC50 value of 7 µM.Formal...

This Ribosomal Protein S6 (Phospho-Ser235/236) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. This Ribosomal Protein S6 (Phospho-Ser235/236) TR-FRET Assay Kit is robust, with a Z' value of 0.52, and suitable for screening a large...