9 products were found matching "Cay700406"!

Cayman's Glycogen Assay provides a simple, reproducible, and sensitive tool for measuring glycogen in tissue/cells. Glycogen is hydrolyzed by amyloglucosidase to form beta-D-glucose, which is then specifically oxidized by glucose oxidase to form hydrogen peroxide. Hydrogen peroxide, in the presence of horseradish...

The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate oxidation are fed into the tricarboxylic acid (TCA or citric acid) cycle. As a mitochondrial...

Cayman's Pyruvate Assay provides both a fluorescence- and absorbance-based methods for quantifying pyruvate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular pyruvate concentrations in cell culture samples. In the assay,...

Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately, oxidation of succinate...

The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman's Thiol Detection Assay Kit provides a simple, reproducible, and sensitive tool for...

Cayman's beta-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying beta-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for beta-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme...

Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans. Therefore, humans must obtain ascorbate in their diet in order to survive. In humans, ascorbate acts as an electron donor for eight different enzymes. It also...

Cayman's Creatinine (serum) Colorimetric Assay can be used to measure creatinine levels in plasma and serum. The assay relies on the Jaffe' reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate.2 The rate of color development is directly proportional to the concentration...

Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity. Cayman's Lipase...