L3MBTL1 Inhibitor Screening Assay Kit

The L3MBTL1 Inhibitor Screening Assay Kit is designed to measure the inhibition of L3MBTL1 binding to its substrate. The kit comes in a convenient AlphaLISA(R) format, with enough biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged L3MBTL1 MBT domain to perform a total of 384 enzyme reactions. The key to the kit is the specific binding of the L3MBTL1 MBT domain to the methylated-peptide substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing L3MBTL1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.