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JMJD3 chemiluminescent assay kit
Gene Regul. homog. Luminometr. Immunological Enzyme kinetics
HTSErklärungen der Abkürzungen ansehen.
BPS
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BPS-​50406 96 rxns

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The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3 is a JmjC-​domain protein that exhibits demethylation activity toward di-​ and trimethyl-​lysine 27 (H3K27me2/3) on histone H3. The JMJD3 Assay Kit comes in a convenient format, with 8-​well strips precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-​labeled antibody, demethylase assay buffer, and purified JMJD3 for 100 enzyme reactions. The key to the JMJD3 Direct Activity Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-​labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-​specific demethylase activity may be detected. Components: JMJD3-​4 µg, primary antibody 6-​25 µl, secondary HRP-​labeled antibody 2-​ 10 µL, 4x JMJD3 assay buffer 3 ml, blocking buffer 50 ml, HRP chemiluminescent substrate 6 ml ea (2 components), 12 x 8-​well strips

Datenblatt ansehen
Manual
Kat #:
BPS-50406
Menge:
96 rxns

Buy Bulk


Preis:
669 €

UniProt ID: O15054 (Protein knowledgebase)

Schlagworte:
JMJD3, KDM6B, EC=1.14.11.-​, Lysine demethylase 6B, Lysine-​specific demethylase 6B, JmjC domain-​containing protein 3, Jumonji domain-​containing protein 3

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