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JMJD3 chemiluminescent assay kit

ART NR.: BPS-50406 (96 rxns)

Hersteller: BPS

Datenblatt



Manual

Anwendungen: Enzyme kinetics, HTS

Format: homog.

UniProt ID: O15054
Suche mit UniProt ID

Geninformationen: GeneID 23135

Schlagworte: JMJD3, KDM6B, EC=1.14.11.-, Lysine demethylase 6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3

96 rxns

669 €

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Bemerkungen: The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3. The JMJD3 Assay Kit comes in a convenient format, with 8-well strips precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD3 for 100 enzyme reactions. The key to the JMJD3 Direct Activity Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-specific demethylase activity may be detected. Components: JMJD3-4 µg, primary antibody 6-25 µl, secondary HRP-labeled antibody 2- 10 µL, 4x JMJD3 assay buffer 3 ml, blocking buffer 50 ml, HRP chemiluminescent substrate 6 ml ea (2 components), 12 x 8-well strips

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