Caymans JMJD2A Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening JMJD2A inhibitors. The assay is based on the multistep reaction in which JMJD2A first produces formaldehyde during the demethylation of the trimethylated peptide substrate, histone H3 trimethyl lys9, with the concomitant oxidation/decarboxylation of 2-oxoglutarate. The detection reaction involves the cyclization between formaldehyde and acetoacetanilide in the presence of ammonia. The resulting fluorescent product is analyzed using the excitation wavelength between 365-375 nm and an emission wavelength between 465-475 nm.
Datenblatt ansehen
Manual
Kat #: Cay700360-96
Menge: 96 wellsBuy Bulk
Preis: 413 €
UniProt ID: O75164 (Protein knowledgebase)
Schlagworte:
KDM4A, JHDM3A, EC=1.14.11.-, Lysine-specific demethylase 4A, Jumonji domain-containing protein 2A, JmjC domain-containing histone demethylation protein 3A
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| Weitere Produkte für dieses Protein:Antibodies
- Anti-JMJD2A, Kat #: A300-861A
- Anti-JMJD2A, Kat #: A300-860A
- Anti-JMJD2A (IHC), Kat #: IHC-00188
- Anti-JMJD2A (N-term), Kat #: WA-AP1046a
- Anti-JMJD2A, NT (JMJD2A, JHDM3A, JMJD2, KIAA0677, Lysine-specific Demethylase 4A, JmjC Domain-containing Histone Demethylation Protein 3A, Jumonji domain-Containing Protein 2A, KDM4A), Kat #: J7876-45D.100
- Anti-Jumonji Domain Containing 2A, Kat #: Cay10382-1
- Anti-Jumonji Domain Containing 2A (JMJD2A, JHDM3A, KIAA0, Kat #: J7876-45.50
- Anti-Jumonji Domain Containing 2A (JMJD2A, JMJD2, JmjC Domain Containing Histone Demethylation Protein 3A, Jumonji C Domain Containing Histone Demethylase 3A, JHDM3A, KDM4A, KIAA0677), Kat #: J7876-45F.100
- Anti-KDM4A (C), Kat #: AC-R0229-2
- Anti-KDM4A, clone 5H1, Kat #: Pro-30087
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Enzymes & Proteins
- JMJD2A(KDM4A) Active Human Recombinant Protein (1-350), Kat #: BPS-50123
- JMJD2A, human recombinant, Kat #: Cay10336-25
- JMJD2A, human recombinant, Kat #: Cay10336-50
- JMJD2A, human recombinant, Kat #: Cay10336-100
- JMJD2A-Strep tagged (human recombinant), Kat #: Cay11299-50
- JMJD2A-Strep tagged (human recombinant), Kat #: Cay11299-100
- JMJD2A-Strep tagged (human recombinant), Kat #: Cay11299-25
siRNA
ELISA and Assay Kits
Peptides
Products for: JMJD2, JHDM3 - jumonji domain-containing protein 2 [EC:1.14.11.-]
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