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JMJD2A Inhibitor Screening Assay Kit

ART NR.: Cay700360-96 (96 wells)

Hersteller: Cayman




Anwendungen: Demethylation assays, JMJD2A inhibitor screening

Messtechnik: Fluorometric

Lagerung: -80°C

Versand: -80°C

UniProt ID: O75164 | Suche mit UniProt ID

KEGG ID: K06709 | Suche mit KEGG ID

Schlagworte: KDM4A, JHDM3A, EC=1.14.11.-, Lysine-specific demethylase 4A, Jumonji domain-containing protein 2A, JmjC domain-containing histone demethylation protein 3A



443 €

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Bemerkungen: Caymans JMJD2A Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening JMJD2A inhibitors. The assay is based on the multistep reaction in which JMJD2A first produces formaldehyde during the demethylation of the trimethylated peptide substrate, histone H3 trimethyl lys9, with the concomitant oxidation/decarboxylation of 2-oxoglutarate. The detection reaction involves the cyclization between formaldehyde and acetoacetanilide in the presence of ammonia. The resulting fluorescent product is analyzed using the excitation wavelength between 365-375 nm and an emission wavelength between 465-475 nm.
Image Art Cay700360-96 Cayman JMJD2A Inhibitor Screening Assay Kit

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