Interleukin-17A/F Heterodimer (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocy

Source: , Two DNA sequences encoding the mature mouse IL-17A (Thr 22-Ala 158, Accession #Q62386) and mature mouse IL-17F (Arg 21-Ala 153, Accession # AAM77568) were expressed separately in E. coli. Molecular Mass: The recombinant mouse IL1-7A/IL-17F is a refolded disulfide-linked heterodimer of IL-17A and , IL-17F. The non-methionyl form of rhIL-17A monomer has a predicted molecular mass of 14.9kD whereas the methionyl form of rhIL1-7F monomer has a predicted molecular mass of 15.5kD. Activity: Measured by its ability to stimulate induce IL-6 production by NIH/3T3 cells (Yao, Z. et al., 1995, Immunity 3:811). The ED 50 for this effect is typically 0.075-0.375ug/ml. , Endotoxin: <1.0 EU/1ug (LAL), Storage and Stability: 12 months from date of receipt, 20 to 70°C as supplied., 1 month, 2 to 8°C under sterile conditions after reconstitution., 3 months, 20 to 70°C under sterile conditions after reconstitution., Use a manual defrost freezer and avoid repeated freeze-thaw cycles.