Interferon Regulatory Factor-3, human recombinant (rHuIRF-3)

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. (www.uniprot.org) Produced in E.coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids (1-112). IRF-3 plays a crucial role in activation of innate immunity and inflammation in response to viral infection.