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Hsp90alpha assay kit

ART NR.: BPS-50293 (100 rxns)

Hersteller: BPS

Datenblatt



Manual

Anwendungen: Enzyme kinetics, inhibitor screening, HTS

Messtechnik: Fluor. Polar.

UniProt ID: P07900
Suche mit UniProt ID

Geninformation: NM_005348

Schlagworte: HSP86, HSP90A, HSP 86, HSP90AA1, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, Renal carcinoma antigen NY-REN-38

100 rxns

440 €

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Bemerkungen: The Hsp90alpha Assay Kit is designed for identification of Hsp90alpha inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant Hsp90alpha. Hsp90alpha is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90alpha functions has been shown to play a role in tumorigenesis and disease progression. The Hsp90alpha inhibitor screening assay kit comes in a convenient 96-well format, with purified Hsp90alpha enzyme, fluorescently labeled geldanamycin and Hsp90alpha assay buffer for 100 enzyme reactions. The key to the Hsp90alpha Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for Hsp90alpha reactions. The fluorescently labeled geldanamycin is incubated with a sample containing Hsp90alpha enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

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