Histone H4, acetylated, Immunoprecipitation Assay Kit, BioAssay,

The assay kit contains reagents optimized for immunoprecipitation of transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor specific primers or probe, is recommended. Applications: immunoprecipitation of chromatin using anti-acetyl-Histone H4 , , Capacity:, 22 assays , , Components: , Anti-acetyl-Histone H4 , Salmon Sperm DNA/Protein A Agarose , all buffers and solutions , Other components required but not included as part of kit are: Reagents Equipment,  Cells, stimulated or treated as appropriate for theexperimental system,  37% Formaldehyde,  PBS,  Dry ice,  Elution buffer: 1% SDS, 0.1M NaHCO3,  Molecular Biology grade Proteinase K,  Glycogen or tRNA,  50% Phenol/50%Chloroform (containing 1% isoamyl alcohol),  > 95% Ethanol,  70% Ethanol,  vortex mixer,  rotating wheel/platform,  shaking incubator,  timer,  variable volume (5-1000ul) pipetters + tips,  microfuge,  variable temperature water bath,  humidified 37ºC incubator,  cell scraper,  sonicator, Storage and Stability: Stable for 1 year at 4°C from date of shipment.