Histone H3(K9) Universal Methyltransferase Assay Kit

The Histone H3(K9) Universal Methyltransferase Assay Kit is designed for the detection of histone H3(K9) methyltransferase (HMT) activity using purified enzymes or cell extracts. Histone H3(K9) methyltransferases are enzymes that catalyze the transfer of a methyl group from the cofactor S-adenosylmethionine to lysine 9 residue of histone H3. The Histone H3(K9) Universal Methyltransferase Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRPlabeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified G9a, SUV39H1 and SUV39H2 enzymes for 100 enzyme reactions. The key to the Histone H3(K9) Universal Methyltransferase Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.nome Biology 2005, 6:227.