Buffer essential for use with the HDAC assay of purified HDACs. Usage: In a 50ul reaction, assay HDAC activity using HDAC (enzyme, lysate, etc.), substrate, HDAC assay buffer, and test inhibitor. Incubate microtiter plate at 37°C for 30 minutes. Add an equal volume (50ul) of HDAC Assay Developer to each well....

HDAC assay developer is an essential reagent for signal generation in HDAC or sirtuin assays done with any of HDAC fluorometric substrates. An appropriate fluorometric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract,...