- Search results for H5109-62
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19 products were found matching "H5109-62"!
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Item number: H5109-60.1
Kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAc (histone deacetylase) activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control....
Application: | HDAC assay |
1,047.00€
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Item number: H5109-66A.20
Buffer essential for use with the HDAC assay of purified HDACs. Usage: In a 50ul reaction, assay HDAC activity using HDAC (enzyme, lysate, etc.), substrate, HDAC assay buffer, and test inhibitor. Incubate microtiter plate at 37°C for 30 minutes. Add an equal volume (50ul) of HDAC Assay Developer to each well....
Application: | Enzyme assays |
485.00€
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Item number: H5109-66B.6
HDAC assay developer is an essential reagent for signal generation in HDAC or sirtuin assays done with any of HDAC fluorometric substrates. An appropriate fluorometric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract,...
Application: | Enzyme assays |
742.00€
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Item number: H5109-46.100
The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins and...
Application: | WB |
Host: | Rabbit |
Species reactivity: | human |
682.00€
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Item number: H5109-53C.50
Recombinant protein corresponding to full length human HDAC8 with a C-terminal His-tag expressed in baculovirus in Sf21 insect cells (NM_018486). Specific Activity: ~290pmol/min/ug , Assay Conditions: , 25mM Tris-HCl, pH 8.0, 137mM sodium chloride, 2.7mM KCl, 1mM MgCl2, 0.05% Tween 20 and 20uM HDAC class 2a...
Keywords: | HD8, HDAC8, CDA07, EC=3.5.1.98, Histone deacetylase 8 |
1,151.00€
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Item number: H5109-10.250
In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form...
Application: | ELISA, IP, WB |
Host: | Sheep |
Species reactivity: | bovine, human |
470.00€
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Item number: H5109-48.100
The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins and...
Application: | ELISA, ICC, IHC, WB |
Host: | Rabbit |
Species reactivity: | human |
682.00€
*
Item number: H5109-49.100
The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins and target...
Application: | ELISA, WB |
Host: | Rabbit |
Species reactivity: | human |
682.00€
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Item number: H5109-55.100
A novel class II histone deacetylase, HDAC10 is most similar to HDAC6. Both contain a unique, putative second catalytic domain not found in other HDACs. In HDAC10, however, this domain is not functional. Unlike HDAC6, which is normally a cytoplasmic deacetylase, HDAC10 resides in both the nucleus and cytoplasm. Data...
Application: | WB |
Host: | Rabbit |
Species reactivity: | human |
From 356.00€
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Item number: H5109-58.50
Applications:, Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:200, Western Blot: 1:500-1:1,000 , Immunohistochemistry: 1:50-1:100 , Optimal dilutions to be determined by the researcher. Storage and...
Application: | IF, IHC, WB |
Host: | Rabbit |
Species reactivity: | human, mouse |
425.00€
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Item number: H5109-47B.200
Applications: , Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: Reported by an independent laboratory to show positive immunostaining for HDAC2 in NIH/3T3 and U2OS cells fixed with paraformaldehyde. Western Blot:...
Keywords: | EC=3.5.1.98, Anti-Histone deacetylase 2 |
Application: | ICC, IP, WB |
Host: | Mouse |
Species reactivity: | bovine, hamster, human, mouse |
985.00€
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Item number: H5109-48B.100
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years...
Application: | WB |
Host: | Rabbit |
Species reactivity: | human |
688.00€
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