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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
H5109-60.1 | 1 kit | - | - |
3 - 19 business days* |
1,047.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with... more
Product information "Histone Deacetylase Assay Kit (Histone H4 Peptide Substrate), BioAssay"
Kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAc (histone deacetylase) activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAc inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity. Quantity: Approximately 100 assays per 100ug of labeled peptide (the exact amount will vary according to labeling efficiency). Use of 5000-10,000 CPM of [3H]-acetyl histone H4 for each assay is recommended. , Quality Control Testing and Research Applications:, [3H]-acetylation of Histone H4 peptide: 70% of [3H]-acetate was incorporated into 100ug Histone H4 peptide, HDAc Assay: Experiment 1: Dependence of [3H]-acetate release (HDAc Activity) on the amount of HeLa Nuclear Extract. Increasing amounts of HeLa nuclear extract were incubated at room temperature with [3H]-acetyl-Histone H4 peptide (10,000CPM). Released [3H]-acetate was measured using a scintillation counter. HeLa nuclear extract is a source of HDAc activity. Experiment 2: Inhibition of [3H]-acetate release (HDAc Activity) by Sodium Butyrate [3H]-acetyl-Histone H4 peptide was incubated overnight at room temperature with 20ug HeLa nuclear extract in the presence or absence of Sodium Butyrate. 250mM Sodium Butyrate inhibited release of [3H]-acetate by ~96%. , Storage and Stability: After receipt, store individual components at temperatures recommended above and on component labels. This kit is stable for 6 months from date of shipment.
Supplier: | United States Biological |
Supplier-Nr: | H5109-60 |
Properties
Application: | HDAC assay |
Format: | Homog. |
Database Information
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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