Heme Oxygenase-2, Recombinant, Human, Western Blot Positive Control (HO-2, Heme Oxygenase 2, HMOX2,

Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway. Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator, biliverdin and its product bilirubin are potent antioxidants, "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs. To date, 3 forms of heme oxygenases (HO1-3) have been identified. HO-2 (mouse/rat 315aa, human 316aa, chromosome 16) is non-inducible form. Except for corticosterone, no other inducer of HO-2 has been identified to date. HO-2 has been localized to endothelial cells and adventitial nerves of blood vessels. It is also localized to neurons in autonomic ganglia, including the petrosal, superior cervical, and nodose ganglia, as well as ganglia in the myenteric plexus of the intestine. Low levels of Ho- 2 are found in most tissues except spleen. At the aa level, HO-1 and HO-2 are 42% homologous. However, each isozyme is evolutionary highly conserved. The molecular weight of mammalian HO-1 is ~31-33kD and HO-2 is ~36kD. Source: Recombinant human HO-2 (1-291aa without TM domain), expressed in E.coli. Applications: Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane, SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.