HUMAN IgM Fab µ fragment

HUMAN IgM (myeloma) Fab µ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum.    No reaction was observed against anti-Human IgG F(c).  Some light chain cross reactivity will occur with anti-Human IgG. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition, alpha and gamma contain approximately 450 amino acids, while µ and epsilon have approximately 550 amino acids. Human IgM Fab mu is ideal for investigators involved in serum protein component research.