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BM-HL007R010SG HOPE® Trial-​Kit Biomol GmbH
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manual
1 kit

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96 €

HOPE® Trial-Kit


Tissue fixation, IHC, ISH Erklärungen der Abkürzungen ansehen.

For easy starting we offer a HOPE® Trail Kit with 100 ml HOPE® I, 1ml HOPE® II and 500 g Low Melting Paraffin sufficient for fixation of 20 tissue samples. HOPE® = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation, including subsequent paraffin-embedding and sectioning that allows the complete analysis of all types of human or animal soft tissues. HOPE is a combination of amino acids and glucose blended together with an organic buffer. For fixing tissue according to the HOPE® standard fixation protocol, HOPE® I and HOPE® II solution (catalog nr. BM-HL002C001), acetone and Low Melting Point Paraffin (52-54°C, catalog nr. BM-PL003S2K ) are required. During HOPE® fixation, tissue samples are incubated in HOPE® I solution, then in HOPE® II solution, followed by a dehydration with acetone and subsequent embedding in low melting paraffin. No alcohols or aldehydes (formalin, glutaraldehyde) are used. This technique avoids cross-linking of macro- molecular structures during the process which opens up new opportunities to characterize tissues out of paraffin blocks. As a result many 'cryo-type' antibodies are working on HOPE® fixed tissue sections and many antibodies are working without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections. There is no 'over-fixation' with HOPE®, as is known with formalin, when keeping the tissue speci

- Manual



Schlagworte:
Formalin-free tissue fixation, HOPE® Fixation, Paraffin-embedding

 

 

Die Preise verstehen sich exkl. Versandkosten, eventuell anfallender Kühlmittelpauschale sowie der jeweils geltenden Mehrwertsteuer
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