HIF-1alpha Transcription Factor Assay Kit

HIF-1alpha Transcription Factor Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10006910-96 96 wells -

6 - 10 business days*

661.00€
 
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix... more
Product information "HIF-1alpha Transcription Factor Assay Kit"
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1alpha has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia. Cayman's HIF-1alpha Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1alpha response element is immobilized to the wells of a 96-well plate. HIF-1alpha contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1alpha response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.Synonyms: ARNT-interacting Protein, Hypoxia-Inducible Factor-1alpha. Origin: Animal/Bovine, Animal/Rabbit, Animal/Goat.
Keywords: Hypoxia-Inducible Factor-1alpha
Supplier: Cayman Chemical
Supplier-Nr: 10006910

Properties

Application: HIF DNA binding
Species reactivity: human, mouse, rat
Format: Solid Phase

Database Information

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H360
P Phrases: P201, P202, P280, P308+P313, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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