H-89 (hydrochloride)

Protein kinase A (PKA) regulates multiple signal transduction events via protein phosphorylation and is integral to all cellular responses involving the cyclic AMP second messenger system. H-89 is a potent, cell permeable inhibitor of PKA that demonstrates an IC50 value of 0.14 µM and a Ki value of 48 nM in standard kinase assays. While widely used to disrupt PKA signaling, the inhibitory activity of H-89 is non-selective. H-89 also inhibits S6K1, MSK1, ROCK2, PKBalpha, and MAPKAP-K1b with IC50 values of 0.08, 0.12, 0.27, 2.6, and 2.8 µM, respectively.Formal Name: N-[2-[[3-(4-bromophenyl)-2-propen-1-yl]amino]ethyl]5-isoquinolinesulfonamide, dihydrochloride. CAS Number: 130964-39-5. Synonyms: 5-Isoquinolinesulfonamide, Protein Kinase Inhibitor H-89. Molecular Formula: C20H20BrN3O2S . 2HCl. Formula Weight: 519.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml, DMSO: 25 mg/ml, Ethanol: 0.15 mg/ml. SMILES: O=S(C1=CC=CC2=CN=CC=C21)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O.Cl.Cl. InChi Code: InChI=1S/C20H20BrN3O2S.2ClH/c21-18-8-6-16(7-9-18)3-2-11-22-13-14-24-27(25,26)20-5-1-4-17-15-23-12-10-19(17)20,,/h1-10,12,15,22,24H,11,13-14H2,2*1H/b3-2+,,. InChi Key: GELOGQJVGPIKAM-WTVBWJGASA-N.