Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human recombinant (rHuGM-CSF)

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human recombinant (rHuGM-CSF)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G8950-10B.2 2 µg - -

3 - 19 business days*

598.00€
G8950-10B.10 10 µg - -

3 - 19 business days*

757.00€
 
Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain... more
Product information "Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human recombinant (rHuGM-CSF)"
Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. Recombinant Human GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques. , Amino acid sequence:, The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically. Biological Activity: Recombinant GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1ng/ml, corresponding to a Specific Activity of 1x10e7 IU/mg. Protein content: Protein quantitation was carried out by two independent methods: , 1. UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). , 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O or buffer of choice and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Csf2, Csfgm, Colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor
Supplier: United States Biological
Supplier-Nr: G8950-10B

Properties

Application: ELISA
Conjugate: No
Host: Insect cells
Species reactivity: human
Format: Highly Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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