GeneAll(R) Expin(TM) Gel SV, mini (50 preps)
DNA fragment purification
For gel extraction of DNA fragments from agarose gel. Complete kit containing columns, collection tubes, buffers, pH indicator in binding buffer and protocol handbook. Ready to use. Spin column/ vacuum column format, no need for organic solvents. The Expin(TM) Gel SV kits provide a rapid and convenient procedure for DNA extraction from standard and low-melting agarose (TAE, TBE). DNA extraction from agarose gel is a common technique for isolation of specific fragments from reaction mixtures. However, the conventional methods either fail to completely remove agarose, shear the DNA or result in low yields. Gel SV kit takes advantage of glass fiber membrane to recover DNA of 80 bp to 10 kb from most grades of agarose gel in yields reaching 90 %. Expin(TM) Gel SV kit has a binding capacity of 10 µg and a minimum elution volume of 30 µl. It is recommended for sample volumes of 200mg up to a maximum of 400 mg and has an average preperation time of 15 minutes. With this kit high purity of A260/A280: 1.8 - 2.0 can be achieved and ethidium bromide is removed completely. The extracted DNA can be used directly for ligation, sequencing, labeling, PCR, enzyme assay or other enzymatic reactions.
Fragment DNA preparation
Datenbank vom 17.05.2013, 09:06 Uhr
Die Preise verstehen sich exkl. Versandkosten, eventuell anfallender Kühlmittelpauschale sowie der jeweils geltenden Mehrwertsteuer