GeneAll(R) Exfection(TM) Plasmid EF (Very Low Endotoxin), midi (100 preps)
For preparation of maximum pure plasmid DNA (Endotoxin level < 0.1 EU/µg). Complete kit containing columns, filter, collection tubes, buffers, RNAse A and protocol handbook. Ready to use. Spin column format, no need for organic solvents. The Exfection(TM) plasmid EF kits provide a simple and fast method for the purification of plasmid DNA with extremely low endotoxin contaminants. Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. The Exfection(TM) kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. With this kit endotoxin levels can thus be reduced to 0.1 EU/µg. The 'Exfection(TM) EF kit, midi' has a binding capacity of 300 µg per column and is recommended for sample volumes of 30 - 150 ml LB. Preparation time is <70 min, minimum elution volume is 500 µl. The purified DNA can be used for all applications that have been described for the 'Plasmid LE' series plus cell transfection of primary , sensitive and/or suspension cell lines, gene silencing and microinjection.