GUINEA PIG IgG Fab fragment

Guinea Pig IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Guinea Pig IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, anti-Guinea Pig IgG and anti-Guinea Pig IgG F(ab')2.  No reaction was observed against anti-Guinea Pig IgG F(c) or anti- Papain. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.