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G9a Direct Activity Assay Kit
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BPS
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BPS-​52044

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96 rxns eingest.

Replacement for BPS-​52001L. The G9a Direct Activity Assay Kit is designed to measure G9a activity using purified G9a or cell extracts containing G9a. The G9a Direct Activity Assay Kit comes in a convenient format, with a 96-​well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-​labeled antibody, S-​adenosylmethionine, methyltransferase assay buffer, and purified G9a enzyme for 100 enzyme reactions. The key to the G9a Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-​adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-​labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. COMPONENTS: 4x HMT assay buffer (3 ml), Blocking buffer (50 ml), 400 µM S-​adenosylmethionine (250 µl), HRP-​labeled antibody 1 (100 µl), HRP chemiluminescent substrate (2 components) 6 ml each, G9a (500 ng), 1 black plate precoated with histone substrate.

Datenblatt ansehen
Manual
Kat #:
BPS-52044
Menge:
96 rxns
eingest.

UniProt ID: Q96KQ7 (Protein knowledgebase)

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Schlagworte:
BAT8, EHMT2, EC=2.1.1.-​, Protein G9a, EC=2.1.1.43, H3-​K9-​HMTase 3, Lysine N-​methyltransferase 1C, HLA-​B-​associated transcript 8, Histone H3-​K9 methyltransferase 3, Histone-​lysine N-​methyltransferase EHMT2

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Products for: EHMT - euchromatic histone-lysine N-methyltransferase [EC:2.1.1.43]




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