Replacement for BPS-52001L. The G9a Direct Activity Assay Kit is designed to measure G9a activity using purified G9a or cell extracts containing G9a. The G9a Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified G9a enzyme for 100 enzyme reactions. The key to the G9a Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. COMPONENTS: 4x HMT assay buffer (3 ml), Blocking buffer (50 ml), 400 µM S-adenosylmethionine (250 µl), HRP-labeled antibody 1 (100 µl), HRP chemiluminescent substrate (2 components) 6 ml each, G9a (500 ng), 1 black plate precoated with histone substrate.
Datenblatt ansehen
Manual
Kat #: BPS-52044
Menge: 96 rxns
eingest.
UniProt ID: Q96KQ7 (Protein knowledgebase)
Passende, verwandte Produkte
Schlagworte:
BAT8, EHMT2, EC=2.1.1.-, Protein G9a, EC=2.1.1.43, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase EHMT2 Loading ...
Relevant Pathways:
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| Weitere Produkte für dieses Protein:Antibodies
- Anti-EHMT2 (M), Kat #: AC-R0438-3
- Anti-EHMT2 (N-term), Kat #: WA-AP5526a
- Anti-G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/, Kat #: G1009-02A.100
- Anti-G9a, ID (Histone-lysine N-methyltransferase, H3 Lysine-9 Specific 3, Histone H3-K9 Methyltransferase 3, H3-K9-HMTase 3, Euchromatic Histone-lysine N-methyltransferase 2, HLA-B Associated Transcript 8, G9a Protein, EHMT2, Lysine N-methyltransferase 1C, Kat #: G1009-02D.100
- Anti-G9a, NT (Histone-lysine N-methyltransferase, H3 Lysine-9 Specific 3, Histone H3-K9 Methyltransferase 3, H3-K9-HMTase 3, Euchromatic Histone-lysine N-methyltransferase 2, HLA-B Associated Transcript 8, G9a Protein, EHMT2, Lysine N-methyltransferase 1C, Kat #: G1009-02C.100
- Anti-G9A/EHMT2, Kat #: A300-933A
- Anti-G9A/EHMT2 (IHC), Kat #: IHC-00489
- Anti-KMT1C / G9a / BAT8 (Center), Kat #: WA-AP1197c
Enzymes & Proteins
- G9a (expressed Sf9), active human recombinant protein, Kat #: BPS-51001
- G9a (human recombinant), Kat #: Cay10353-25
- G9a (human recombinant), Kat #: Cay10353-100
- G9a (human recombinant), Kat #: Cay10353-50
- G9a, active human recombinant protein (expressed E. coli), Kat #: BPS-51000
- G9a, active, Recombinant, Human (SET Domain-containing Protein, G9a Histone Methyltransferase, G9a HMTases), Kat #: G1009-01.50
siRNA
ELISA and Assay Kits
Primers
Peptides
Products for: EHMT - euchromatic histone-lysine N-methyltransferase [EC:2.1.1.43]
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