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G9a Direct Activity Assay Kit

ART NR.: BPS-52044 (96 rxns)

Hersteller: BPS



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Anwendungen: Enzyme kinetics, inhibitor screening, HTS

Messtechnik: Luminometric

Lagerung: Various

Versand: -80°C

UniProt ID: Q96KQ7
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Geninformationen: GeneID 10919

Schlagworte: BAT8, EHMT2, EC=2.1.1.-, Protein G9a, EC=, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase EHMT2


Bemerkungen: Replacement for BPS-52001L. The G9a Direct Activity Assay Kit is designed to measure G9a activity using purified G9a or cell extracts containing G9a. The G9a Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified G9a enzyme for 100 enzyme reactions. The key to the G9a Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. COMPONENTS: 4x HMT assay buffer (3 ml), Blocking buffer (50 ml), 400 µM S-adenosylmethionine (250 µl), HRP-labeled antibody 1 (100 µl), HRP chemiluminescent substrate (2 components) 6 ml each, G9a (500 ng), 1 black plate precoated with histone substrate.


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Products for: EHMT - euchromatic histone-lysine N-methyltransferase [EC:]

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