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![Fluo-8FF(TM), AM](https://www.biomol.com/media/image/8d/a9/ec/ABD-21105_600x600.gif)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
ABD-21104 | 500 µg (10 x 50 µg) | - |
3 - 8 business days* |
279.00€
|
|||
ABD-21105 | 1 mg | - |
3 - 8 business days* |
418.00€
|
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that... more
Product information "Fluo-8FF(TM), AM"
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8(R) dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Fluo-8(R) AM only requires room temperature while Fluo-3 AM and Fluo-4 AM require 37 ?C cell loading. In addition, Fluo-8(R) is 2 times brighter than Fluo-4 AM, and 4 times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8(R) reagents with different calcium binding affinities (Fluo-8(R): Kd = 389 nM, Fluo-8H: Kd = 232 nM, Fluo-8L: Kd = 1.86 µM, Fluo-8FF: Kd = 10 µM). We also offer versatile packing sizes to meet your special needs, e.g., 1 mg, 10x50 µg, 20x50 µg, HTS packages with no additional packaging charge.
Supplier: | AAT Bioquest |
Supplier-Nr: | 21104 |
Properties
Application: | IF, FC, Fluorescent Ca2+ probe |
MW: | ~1100 D |
Database Information
Handling & Safety
Storage: | -20°C (protect from light) |
Shipping: | +20°C (International: +20°C) |
GHS Hazard Pictograms: |
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H Phrases: | H302, H312, H332 |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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