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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E3374-03D.100 | 100 µg | - | - |
3 - 19 business days* |
416.00€
|
||
E3374-03D.500 | 500 µg | - | - |
3 - 19 business days* |
606.00€
|
Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino... more
Product information "Epidermal Growth Factor (EGF), human recombinant (rHuEGF)"
Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli. AA Sequence: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR, Biological activity: The ED50 for this effect is < 0.89 ng/ml . Measured in a cell proliferation assay using mouse Balb3T3 cell. Activity Assay: 1. Cell line: Balb-3T3 (Mus musculus (mouse)) , 2. Maintenance Condition: RPMI 1640 containing 10% FBS , 3. Assay medium - serum free RPMI 1640 , 4. Cell density: 2x10e4 cells/well (96 well plate, final volume 100ul) , 5. Serum free starvation : 24hr with RPMI1640 , 6. Incubation time : 24 hr (after sample treatment) , 7. Concentration range: 1 pg/ml - 1 ug/ml , 8. Detection method : BrdU assay, Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Epidermal growth factor, Pro-epidermal growth factor |
Supplier: | United States Biological |
Supplier-Nr: | E3374-03D |
Properties
Conjugate: | No |
Host: | E.coli |
Species reactivity: | human |
Format: | Highly Purified |
Database Information
KEGG ID : | K04357 | Matching products |
UniProt ID : | P01133 | Matching products |
Gene ID | GeneID 1950 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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