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EZH2 chemiluminescent assay kit
Gene Regul. solid phase Luminometr. Enzymatic Purified Enzymes Enzyme kinetics
HTSErklärungen der Abkürzungen ansehen.
BPS
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BPS-​52009L 100 rxns

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The EZH2/EED/SUZ12/RbAp48/AEBP2 Direct Activity Assay kit is designed to measure EZH2/EED/SUZ12/RbAp48/AEBP2 activity using purified enzyme complex or EZH2/EED/SUZ12/RbAp48/AEBP2 extracts. The EZH2/EED/SUZ12/RbAp48/AEBP2 Direct Activity Assay Kit comes in a convenient format, with a 96-​well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H27, the secondary Europium-​labeled antibody, S-​adenosylmethionine, methyltransferase assay buffer, and purified EZH2/EED/SUZ12/RbAp48/AEBP2 enzymes for 100 enzyme reactions. The key to the EZH2/EED/SUZ12/RbAp48/AEBP2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K27 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-​adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-​labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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Manual
Kat #:
BPS-52009L
Menge:
100 rxns

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Preis:
1.495 €

UniProt ID: Q15910 (Protein knowledgebase)

Schlagworte:
KMT6, EZH2, ENX-​1, EC=2.1.1.43, Enhancer of zeste homolog 2, Lysine N-​methyltransferase 6, Histone-​lysine N-​methyltransferase EZH2

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