EZH2 (A677G) Chemiluminescent Assay Kit

The EZH2 (A677G) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex [EZH2 (A677G)/EED/SUZ12/RbAp48/AEBP] for screening and profiling purposes. The key to the EZH2 (A677G) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.