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EHMT1/GLP Chemiluminescent Assay Kit

ART NR.: BPS-53007 (96 rxns)

Hersteller: BPS

Datenblatt



Manual

Anwendungen: EHMT1 activity screening

UniProt ID: Q9H9B1
Suche mit UniProt ID

Geninformationen: GeneID 79813

Schlagworte: GLP, GLP1, EHMT1, EUHMTASE1, Eu-HMTase1, EC=2.1.1.-, EC=2.1.1.43, H3-K9-HMTase 5, G9a-like protein 1, Lysine N-methyltransferase 1D, Histone H3-K9 methyltransferase 5, Histone-lysine N-methyltransferase EHMT1

96 rxns

696 €

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Bemerkungen: The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure EHMT1 (also known as G9a-like protein) activity for screening and profiling applications. The EHMT1 (GLP) Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, primary antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EHMT1 for 100 enzyme reactions. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-specific methyltransferase activity may be detected.

Spezifikationen

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