Dithiothreitol (DTT) (1,4-Dithiothreitol)

Dithiothreitol (DTT) (1,4-Dithiothreitol)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
D8070.5 5 g - -

3 - 19 business days*

113.00€
D8070.10 10 g - -

3 - 19 business days*

144.00€
D8070.25 25 g - -

3 - 19 business days*

341.00€
D8070.50 50 g - -

3 - 19 business days*

523.00€
D8070.100 100 g - -

3 - 19 business days*

834.00€
 
Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's... more
Product information "Dithiothreitol (DTT) (1,4-Dithiothreitol)"
Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's oxidized form is a disulfide-bonded 6-membered ring. Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE). DTT is an unusually strong reducing agent, owing to its high conformational propensity to form a six-membered ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions and is illustrated below. The intermediate mixed-disulfide state is unstable (i.e., poorly populated) because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond. The reducing power of DTT is limited to pH values above ~7, since only the negatively charged thiolate form -S- is reactive (the protonated thiol form -SH is not), the pKa of thiol groups is typically ~8.3. , Applications:, A common use of DTT is as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting.", DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. However, even DTT cannot reduce buried (solvent-inaccessible) disulfide bonds, so reduction of disulfide bonds is sometimes carried out under denaturing conditions (e.g., at high temperatures, or in the presence of a strong denaturant such as 6M guanidinium hydrochloride, 8M urea, or 1% sodium dodecylsulfate). Conversely, the solvent exposure of different disulfide bonds can be assayed by their rate of reduction in the presence of DTT. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione. In very rare cases, a DTT adduct may be formed, i.e., the two sulfur atoms of DTT may form disulfide bonds to different sulfur atoms, in such cases, DTT cannot cyclize since it has no remaining free thiols. Due to air oxidation, DTT is a relatively unstable compound whose useful life can be extended by refrigeration and handling in an inert atmosphere. Since protonated sulfurs have lowered nucleophilicities, DTT becomes less potent as the pH lowers. Tris(2-carboxyethyl)phosphine HCl (TCEP hydrochloride) is an alternative which is more stable and works even at low pH. Synonyms: Cleland's reagent,, (2R,3R)-rel-1,4-Dimercapto-2,3-butanediol, (±)-1,4-Dimercapto-2,3-butanediol, (±)-Dithiothreitol, 1,4-Dithio-DL-threitol, 1,4-Dithiothreitol, DL-1,4-Dimercapto-2,3-dihydroxybutane, DL-1,4-Dithiothreitol, DL-Dithiothreitol, Sputolysin, WR 34678, threo-1,4-Dimercapto-2,3-butanediol, threo-2,3-Dihydroxy-1,4-butanedithiol, threo-2,3-Dihydroxy-1,4-dithiolbutane, CAS No: 3483-12-3, Molecular Formula: C4H10O2S2, Molecular Weight: 154.24, Appearance: White crystalline powder, Purity: >99%, Solubility (5%): Clear, colorless and complete, pH (5%): 4.3±0.5, Melting Point: 39.5-44°C, RNase: None Detected, DNase: None Detected, Protease: None Detected, Storage and Stability: Lyophilized DTT powder is hygroscopic and should be stored desiccated at 4ºC. Stable for 6 months after receipt. DTT is not stable in solution. A freshly made DTT solution aliquot should be made prior to each use.
Supplier: United States Biological
Supplier-Nr: D8070

Properties

MW: 154.24 D
Formula: C4H10O2S2
Format: Molecular Biology Grade

Database Information

CAS : 3483-12-3| Matching products

Handling & Safety

Storage: +4°C
Shipping: +20°C (International: +20°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H302, H319, H335
Caution
Our products are for laboratory research use only: Not for administration to humans!
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