DNMT3B/DNMT3L human recombinant protein complex

Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal GST-tag, MW=59 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal His-tag, MW = 27 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system. Assay conditions: 50 ?l reaction mix containing DNMT3B/DNMT3L added to wells coated with the DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection. Application: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.