DNA Marker FX174, BsuRI (HaeIII), 11 Fragments (72-1353bp), BioAssay (Supplied with 6x Loading Dye S

PhiX174 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 11 discrete fragments (in base pairs): 1353, 1078, 872, 603, 310, 281, 271, 234, 194, 118, 72. , 6x Loading Dye Solution (L3350): Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60mM EDTA, 60% glycerol. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Recommendations for Use: I. Loading on Agarose Gel, 1. Prepare DNA Marker before , loading:, 1ul (0.5ug) of D3930-35 DNA Marker, 1ul of L3350 6X Loading Dye Solution, 4ul of deionized water , 2. Vortex gently just prior to use. Do not heat before loading., 3. Apply the prepared amount (6ul) of , the DNA Marker on a 5mm lane , of agarose gel., 4. Following electrophoretic separation , on gel, visualize the DNA bands by , ethidium bromide staining., Use 0.1ug (0.2ul) of the DNA Marker (before dilution) per 1mm of an agarose gel lane width. II. Loading on Polyacrylamide Gel 1. Prepare DNA Marker before , loading:, 2ul (1ug) of D3930-35 DNA Marker, 0.5ul of L3350 6X Loading Dye Solution, 0.5ul of deionized water , 2. Vortex gently just prior to use. Do not heat before loading., 3. Apply the prepared amount (3ul) of , the DNA Marker on a 5mm lane , of polyacrylamide gel., 4. Following electrophoretic separation , on gel, visualize the DNA bands by , ethidium bromide staining., , Use 0.2ug (0.4ul) of the DNA Marker (before dilution) per 1mm of a Polyacrylamide gel lane width. 310, 281, 271 bp bands migrate anomalously.