Cyclic AMP ELISA Kit (without Acetic Anhydride)

Cyclic AMP ELISA Kit (without Acetic Anhydride)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay581002-96S 96 so-wells (96 solid wells) -

6 - 10 business days*

422.00€
Cay581002-96 96 st-wells (96 strip wells) -

6 - 10 business days*

422.00€
Cay581002-480S 480 so-wells (480 solid wells) -

6 - 10 business days*

1,571.00€
Cay581002-480 480 st-wells (480 strip wells) -

6 - 10 business days*

1,571.00€
 
Cayman's cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within... more
Product information "Cyclic AMP ELISA Kit (without Acetic Anhydride)"
Cayman's cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B0) of 0.1 pmol/ml once samples and standards have been acetylated prior to analysis. This assay has been validated with culture media, urine, tissue, and plasma samples. If you prefer to assay your samples and still achieve excellent sensitivity without the need to perform a pre-assay acetylation step we recommend using Cayman's cAMP Select ELISA Kit. Cyclic AMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.Synonyms: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit (without Acetic Anhydride). Assay Range: 0.078-10 pmol/ml. Sensitivity: 80% B/B0: 0.1 pmol/ml. Mid-point: 50% B/B0: 0.5 pmol/ml. Cross Reactivity: (Non-Acetylated cAMP) 100%, (Non-Acetylated cGMP) 1.5%, (Non-Acetylated Adenosine) <0.01%, (Non-Acetylated AMP) <0.01%, (Non-Acetylated ATP) <0.01%, (Acetylated cAMP) 100%, (Acetylated cGMP) 0.69%, (Acetylated Adenosine) <0.01%, (Acetylated AMP) <0.01%. Origin: Animal/Mouse, Animal/Bovine, Animal/Rabbit, Animal/Eel.
Keywords: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit (without Acetic Anhydride)
Supplier: Cayman Chemical
Supplier-Nr: 581002

Properties

Application: ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H290, H301, H302, H311, H314, H315, H318, H319, H335, H350, H373
P Phrases: P201, P202, P234, P260, P261, P264, P270, P271, P280, P312, P314, P321, P363, P390, P301+P310, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P305+P351+P338, P308+P313, P332+P313, P337+P313, P361+P364, P362+P364, P405, P406, P403+P233, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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