Conalbumin, Chicken Egg, Protein Control for Western Blot (Ovotransferrin)

Allergy to chicken egg or proteins is one of the more frequent causes of food hypersensitivity in infants and young children. Both IgG and IgA class antibodies may be detected. Ovalbumin, intolerance has been implicated in a number of conditions affecting children. In particular, children with cystic fibrosis show elevated anti-ovalbumin antibodies. Ovalbumin antibodies have, also been noted in some forms of kidney disease. A relationship between food allergy and infantile autism has also been observed. Children with insulin-dependent diabetes mellitus show an enhanced immune response to both b-lactoglobulin and ovalbumin, a phenomenon that may be related to the development of the disease. Conditions related to ovalbumin intolerance usually resolve once egg and egg based foods have been withdrawn from the patient's diet., Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk. The major proteins of chicken eggs are: Ovalbumin (45 kD, 54%), Conalbumin (13%, 80kD), Ovomucoid(11%, 28kD), Lysozyme (3.5%, 14kD), Globulins (G2, G3) (8.0%, mol wt?), Ovomucin (1.5%, mol wt?). Other protein components include, flavoprotein (0.8%), ovoglycoprotein (0.5%), ovomacroglobulin (0.5%), ovoinhibitor (0.l%) and avidin (0.05%). Conalbumin (Ovotransferrin precursor (Conalbumin) (Allergen Gald 3) (Gal d III) (Serum transferrin) is produced from a precursor of 705 aa. it is found in egg white. Conalbumin or transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further ole in stimulating cell proliferation. Transferrin has a bacteriostatic function. Its concentration in avian egg is the highest concentration of any transferrin in vivo. Transferrin in liver is regulated by the iron levels, but not in the oviduct. These two forms of transferrin (liver and oviduct) differ only by their carbohydrate composition. It causes an allergic reaction in human. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: load 10ul/lane. , Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2X sample buffer per 10ul of the O9050 solution prior to heating and loading on gels. This preparation is not biologically active. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.