Chk2, Human, Recombinant, NT, GST-His tag (Checkpoint Kinase 2, Rad 53)

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both functionas essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Human Check point kinase 2 (CHK2) (GenBank Accession No. NM_007194), full length, with N-terminal GST-HIS6-tag, expressed in an Baculovirus infected Sf9 cell expression system. The theoretical MW is 91kD. , Source: Baculovirus infected Sf9 cells, Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: 1 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of CHKtide substrate per minute at pH 7.4 and 30°C. Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP. Cellular Localization: Nuclear , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.