ChREBP Transcription Factor Assay Kit
ChREBP binding detection
Cayman's ChREBP Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the ChREBP response element is immobilized onto the wells of a 96 well plate. ChREBP contained in a nuclear extract binds specifically to the ChREBP response element. ChREBP is detected by addition of specific primary antibody directed against ChREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.