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Cay10006909-96 ChREBP Transcription Factor Assay Kit Cayman
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96 wells

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476 €

ChREBP Transcription Factor Assay Kit


ChREBP binding detection Erklärungen der Abkürzungen ansehen.

Cayman's ChREBP Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the ChREBP response element is immobilized onto the wells of a 96 well plate. ChREBP contained in a nuclear extract binds specifically to the ChREBP response element. ChREBP is detected by addition of specific primary antibody directed against ChREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

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Image Art 10006909 Cayman ChREBP Transcription Factor Assay Kit




 


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