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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
ABD-21180 | 1000 tests | - |
3 - 8 business days* |
556.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Ex: 503 nm. Em: 528 nm. Intracellular pH change are implicated in diverse physiological and... more
Product information "Cell Meter(TM) Fluorimetric Intracellular pH Assay Kit"
Ex: 503 nm. Em: 528 nm. Intracellular pH change are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders and Alzheimer's disease. The Cell MeterT Fluorimetric Intracellular pH Assay Kit utilizes AAT Bioquest's proprietary fluorescent indicator for measuring the relative intracellular pH changes. It is a homogeneous, kinetic, live-cell fluorescent assay that utilizes either a standard procedure or acid-load procedure. The standard protocol is designed for measuring the therapeutic targets of interest with a decrease in intracellular pH upon treatment. The 'Acid-Load' procedure is designed to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation or growth factor activity. With the 'Acid-Load' procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This 'acid-loading' step is followed by the addition of agonist in a relatively large volume (~4X) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
Supplier: | AAT Bioquest |
Supplier-Nr: | 21180 |
Properties
Application: | Cell-based assays, cell biology, pH and ion indicators |
Format: | Homog. |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +20°C (International: +20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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