Cell Meter(TM) Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation*

There are a variety of parameters that can be used to monitor cell viability. The proprietary violet laser ?excitable fluorescent dye used in the kit is a hydrophobic compound that easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly blue fluorescent product that is well-retained in the cell cytoplasm. The blue fluorophore generated by the esterase hydrolysis of the non-fluorescent substrate has the spectral properties of fluorescein at Ex/Em = approx. 405 nm/450 nm. When excited at 405 nm, the fluoreophore emits intense blue fluorescence at approx. 450 nm. The kit provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. It can also be used with a fluorescence microscope equipped with the Pacific Blue(R) or V450(R) filter set. This Cell Meter(TM) Cell Viability Assay Kit provides an effective tool of labeling cells for fluorescence flow cytometry, microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit is suitable for proliferating and non-proliferating cells.