Cell Meter(TM) Apoptotic and Necrotic Detection Kit *Triple Fluorescence Color*

Our Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to simultaneously monitor apoptotic, necrotic and healthy cells. Apoptosis is described as an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor used in this kit has red fluorescence (Ex/Em = 550/590 nm) upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable DNA Nuclear Green(TM) DCS1 (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye CytoCalcein(TM) Violet 450 (Ex/Em = 405/450 nm) for labeling living cell cytoplasm. This kit is optimized to simultaneously detect cell apoptosis (Red), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.