Cell Explorer(TM) Live Cell Labeling Kit *Green Fluorescence*

Our Cell Explorer(TM) fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells in green fluorescence. The kit uses non-fluorescent Calcein Green(TM) that becomes strongly fluorescent upon entering into live cells. Calcein Green(TM) is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent Calcein Green(TM) by intracellular esterases generates the strongly fluorescent hydrophilic Calcein Green(TM) fluorophore that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.