CBP bromodomain TR-FRET Assay Kit

CBP bromodomain TR-FRET Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay600850-384 384 wells

6 - 10 business days*

769.00€
Cay600850-1920 1920 wells

6 - 10 business days*

2,876.00€
Cay600850-9600 9600 wells

6 - 10 business days*

12,869.00€
 
Cayman's CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance... more
Product information "CBP bromodomain TR-FRET Assay Kit"
Cayman's CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of CBP bromodomain (BRD, human, amino acids 1,081-1,197) directly labeled with a europium (Eu3+) chelate (BRD-Eu3+). A biotinylated peptide containing acetylated lysines serves as the ligand for the CBP bromodomain. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-labeled avidin resulting in a loss of TR-FRET signal. The CBP bromodomain TR-FRET Assay Kit is robust (Z´ = >0.6) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.Excitation: 340 nm. Origin: Animal/Bovine.
Keywords: CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein-lysine acetyltransferase CREBBP
Supplier: Cayman Chemical
Supplier-Nr: 600850

Properties

Application: HTS, TR-FRET

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H302, H315, H316, H317, H319, H335, H373, H412
P Phrases: P260, P261, P264, P270, P271, P272, P273, P280, P301+310, P302+352, P304+340, P305+351+338, P312, P314, P321, P330, P332+313, P333+313, P337+313, P362+364, P403+233, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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