Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Cayman's CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance... more
Product information "CBP bromodomain TR-FRET Assay Kit"
Cayman's CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Förster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of CBP bromodomain (BRD, human, amino acids 1,081-1,197) directly labeled with a europium (Eu3+) chelate (BRD-Eu3+). A biotinylated peptide containing acetylated lysines serves as the ligand for the CBP bromodomain. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-labeled avidin resulting in a loss of TR-FRET signal. The CBP bromodomain TR-FRET Assay Kit is robust (Z´ = >0.6) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO.Excitation: 340 nm. Origin: Animal/Bovine.
Keywords: | CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein-lysine acetyltransferase CREBBP |
Supplier: | Cayman Chemical |
Supplier-Nr: | 600850 |
Properties
Application: | HTS, TR-FRET |
Database Information
KEGG ID : | K04498 | Matching products |
UniProt ID : | Q92793 | Matching products |
Gene ID | GeneID 1387 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -80°C (International: -80°C) |
Signal Word: | Warning |
GHS Hazard Pictograms: |
|
H Phrases: | H302, H315, H316, H317, H319, H335, H373, H412 |
P Phrases: | P260, P261, P264, P270, P271, P272, P273, P280, P301+310, P302+352, P304+340, P305+351+338, P312, P314, P321, P330, P332+313, P333+313, P337+313, P362+364, P403+233, P405, P501 |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed