BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

The BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BTLA:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BTLA is coated on a 96-well plate. Next, HVEM is incubated with BTLA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.