BRD1 Inhibitor Screening Assay Kit

The BRD1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD1 binding to its substrate. The BRD1 Inhibitor Screening Assay Kit comes in a convenient AlphaLISA(R) format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged BRD1 bromodomain to perform a total of 384 enzyme reactions. The key to the BRD1 Inhibitor Screening Assay Kit is the specific binding of the BRD1 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.