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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
B2680-05A.10 | 10 µg | - | - |
3 - 19 business days* |
955.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Human BRAF (a.a. 432-725) with N-terminal His tag, MW 36kD, co-expressed with p50 in an... more
Product information "BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag"
Human BRAF (a.a. 432-725) with N-terminal His tag, MW 36kD, co-expressed with p50 in an Baculovirus infected Sf9 cell expression system. Application: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested. , Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: As reported. Kinase Assay: , Activity is tested using 1uM MEK1 (K97R) as substrate. Assay is performed in a 50ul reation in 10mM Tris, pH 7.4, 5mM magnesium chloride, 0.5mM EDTA, 0.5mM DTT, 5uM ATP, 5ug MEK1 (K97R) as substrate, and B2680-05A. 62ng of BRAF enzyme phosphorylated over 18% of 1uM MEK1 at RT in 1hr. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Keywords: | p94, BRAF1, EC=2.7.11.1, Proto-oncogene B-Raf, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1 |
Supplier: | United States Biological |
Supplier-Nr: | B2680-05A |
Properties
Conjugate: | No |
Species reactivity: | human |
Database Information
KEGG ID : | K04365 | Matching products |
UniProt ID : | P15056 | Matching products |
Gene ID | GeneID 673 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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