BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag

BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag
Item number Size Datasheet Manual SDS Delivery time Quantity Price
B2680-05A.10 10 µg - -

3 - 19 business days*

955.00€
 
Human BRAF (a.a. 432-725) with N-terminal His tag, MW 36kD, co-expressed with p50 in an... more
Product information "BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag"
Human BRAF (a.a. 432-725) with N-terminal His tag, MW 36kD, co-expressed with p50 in an Baculovirus infected Sf9 cell expression system. Application: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested. , Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: As reported. Kinase Assay: , Activity is tested using 1uM MEK1 (K97R) as substrate. Assay is performed in a 50ul reation in 10mM Tris, pH 7.4, 5mM magnesium chloride, 0.5mM EDTA, 0.5mM DTT, 5uM ATP, 5ug MEK1 (K97R) as substrate, and B2680-05A. 62ng of BRAF enzyme phosphorylated over 18% of 1uM MEK1 at RT in 1hr. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Keywords: p94, BRAF1, EC=2.7.11.1, Proto-oncogene B-Raf, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1
Supplier: United States Biological
Supplier-Nr: B2680-05A

Properties

Conjugate: No
Species reactivity: human

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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