Anti-phospho-p53 (Ser33) (Tumor Suppressor Protein, Oncogene Protein)

Anti-phospho-p53 (Ser33) (Tumor Suppressor Protein, Oncogene Protein)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P1001-28.100 100 µl - -

3 - 19 business days*

757.00€
 
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other... more
Product information "Anti-phospho-p53 (Ser33) (Tumor Suppressor Protein, Oncogene Protein)"
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to reduced interaction of p53 with its negative regulator, oncoprotein MDM2 (4). MDM2 inhibits the accumulation of p53 by targeting it for ubiquitination and proteasomal degradation (6,7). p53 can apparently be phosphorylated by ATM, ATR and DNAPK at Ser15 and Ser37, the phosphorylations impair the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage (4,5). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity (8,9). p53 is phosphorylated at Ser392 in vivo (11,12) and by CAK in vitro (12). Phosphorylation of p53 at Ser392 is altered in human tumors (14) and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53 (10,11,13). p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo (10,15). Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis (16). In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV-irradiation has been observed (17,18). Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunocytochemistry: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: P1001-28

Properties

Application: ICC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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