Anti-Hypoxanthine Phosphoribosyltransferase 1 (HPRT1, Hypoxanthine-guanine Phosphoribosyltransferase

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. Applications: Suitable for use in Western Blot, ELISA. Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:500~1600, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.