Anti-Heat Shock Cognate Protein 70, luminal, Endoplasmic Reticulum (HSC70, BiP), clone 4E113

The endoplasmic reticulum (ER) luminal Hsc70 protein, also known as BiP, is a member of the multigene 70kD family of stress proteins which is involved in the proper translocation and processing of secretory proteins passing through the ER. In spinach, BiP is expressed constitutively in all tissues, except dry seeds, and its levels remain constant in response to environmental stresses such as cold acclimation, drought stress or heat shock (2). This is in contrast to spinach BiP mRNA levels, however, which increase during cold acclimation and decrease in response to drought stress or heat shock (2). Low temperatures may alter protein biogenesis resulting in an accumulation of misfolded proteins that are chaperoned by the existing pool of free BiP protein which in turn, signals an up-regulation of BiP mRNA (2). On the other hand, drought stress and heat shock may reduce protein export through the secretory pathway, thus signaling a down-regulation of BiP mRNA (2). In any case, it appears that the level of constitutively expressed BiP protein is sufficient to maintain proper protein biogenesis when faced with environmental stresses (2)., , Applications: , Western Blot (Colorimetric) (2,6): 1ug/ml , Western Blot (ECL) (7) , Immunoprecipitation (5): 10ug/ml , Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.