Anti-Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colo

Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kD glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.The neutralisation dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5ug/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions. Applications: Suitable for use in ELISA, Functional Assays, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 25ug/ml, ELISA: 0.5-1ug/ml, Western Blot: 1-2ug/ml, non­reducing conditions only., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.